Additional file 3 of Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies

Additional file 3: Investigation of DNA analogs induced activation of the IFN-β or ISRE promoter in HEK293 cells. (A) HEK293 cells were transfected with the IFN-β or ISRE luciferase reporter plasmids and β-galactosidase or empty vectors for 24 h. After 24 h of transfection, the cells were stimulated with transfected poly(dA:dT) (1 μg/ mL) for 24 h. A luciferase assay was performed 24 h later. (B) HEK293 cells were stimulated with transfected poly(dA:dT) (1 μg/mL) at the indicated times. The cells were lysed and subjected to immunoblot analysis using the indicated antibodies. β-actin was used as a loading control. The iBright analysis software quantified the band intensities (Invitrogen, Version 5.2.0). The experiments were carried out in triplicates, and the results represent the means ± SD. One-way ANOVA with Tukey’s multiple comparisons test was used to determine the significance level. ***p < 0.001.