Additional file 5 of MMP28 recruits M2-type tumor-associated macrophages through MAPK/JNK signaling pathway-dependent cytokine secretion to promote the malignant progression of pancreatic cancer

Supplementary Material 5. Figure S5. MMP28 induces M2 TAM polarization by promoting amino acid metabolism in TAMs. (A-B) RNA sequencing was performed on TAMs cocultured with control (Vector) and MMP28-overexpressing (OE-MMP28) AsPC-1 cells. Volcano mapping and KEGG pathway enrichment analysis were subsequently performed for differentially expressed genes in TAMs. (C-D) RNA sequencing was performed on TAMs cocultured with AsPC-1 cells of MMP28-overexpressing group (OE-MMP28) and MMP28-overexpressing+MAB208-SP group (OE-MMP28+MAB208-SP), and the differentially expressed genes in TAMs were mapped by volcano plot and analysed by KEGG pathway enrichment. (E-F) RNA sequencing was performed on TAMs cocultured with AsPC-1 cells of the MMP28-overexpressing group (OE-MMP28) and the MMP28-overexpressing+MAB293-SP group (OE-MMP28+MAB293-SP), and the differentially expressed genes were mapped by a volcano plot and analysed by KEGG pathway enrichment. (G-H) The overlap of upregulated differentially expressed in TAMs cocultured with OE-MMP28 cells with downregulated differentially expressed genes in TAMs cocultured with OE-MMP28+MAB208-SP cells identified by RNA sequencing was determined and analysed for KEGG pathway enrichment. (I-J) The overlap of upregulated differentially expressed genes in TAMs cocultured with OE-MMP28 cells with downregulated differentially expressed genes in TAMs cocultured with OE-MMP28+MAB293-SP cells identified by RNA sequencing were taken and analyzed for KEGG pathway enrichment.