Mathematical Model of the Zebrafish Ventricular Cardiomyocyte Action Potential and Calcium Transient

Collection of figures from the manuscript titled <i>“A Mathematical Model of the Zebrafish Cardiac Ventricular Action Potential.”</i> This includes novel experimental restitution data from zebrafish hearts, collected using optical mapping and microelectrode recordings and patch clamp data from the L-type calcium current. All individual data points are plotted in the figures 11, 12, and 13.Excel file containing all raw data.<b>Paper Figures.xlsx</b> contains the data used to generate the plots shown in the manuscript.<b>Patch Clamp Data.xlsx</b> includes the raw data from L-type calcium current (<i>I</i>_<em>CaL</em>) patch clamp experiments.Data related to:<b>Figure 2: </b><b>Steady-state </b><b>(in)activation </b><b>and time constant curves describing the </b><b>voltage (</b><b>V</b><b>m</b><b>)-dependent </b><b>gating</b><b> of </b><b><i>I</i></b>_{<i><strong>N</strong></i>}_{<i><strong>a</strong></i>}<b>. </b><b>Figure</b><b> 3: </b><b>Experimental </b><b>current-voltage</b><b> (</b><b>I-V</b><b>)</b><b> </b><b>data</b><b> from </b><b> </b><b>for </b><b><i>I</i></b>_{<i><strong>C</strong></i>}_{<i><strong>a</strong></i>}_{<i><strong>T</strong></i>}<b>.</b><b>Figure 4: S</b><b>teady-state and time constant curves describing the </b><b>voltage (</b><b>V</b><b>m</b><b>)-dependent </b><b>gating of </b><b><i>I</i></b>_{<i><strong>C</strong></i>}_{<i><strong>a</strong></i>}_{<i><strong>L</strong></i>}<b>. </b><b>Figure 5: </b><b>Steady-state and time constant curves describing the </b><b>voltage (</b><b>V</b><b>m</b><b>)-dependent </b><b>gating of </b><b><i>I</i></b>_{<i><strong>K</strong></i>}_{<i><strong>r</strong></i>}<b>.</b><b>Figure 6: </b><b>Steady-state and time constant curves describing the </b><b>voltage (</b><b>V</b><b>m</b><b>)-dependent</b><b> </b><b><i>I</i></b>_{<i><strong>K</strong></i>}_{<i><strong>s</strong></i>}<b>.</b><b>Figure 7:</b><b> </b><b>Experimental </b><b>current-voltage (I-V) data</b><b> </b><b>and the corresponding </b><b>computational</b><b> I</b><b>-</b><b>V curve for </b><b><i>I</i></b>_{<i><strong>K</strong></i>}_{<i><strong>1</strong></i>}<b>.</b><b> </b><b>Figure 8: </b><b>Comparison of the experimental action potential recordings and 0D and 1D computational outputs for steady-state pacing with a cycle length of 500 </b><b>ms</b><b>.</b><b> </b><b>Figure 9: </b><b>Comparison of the experimental calcium transient recordings and 0D and 1D computational outputs for steady-state pacing with a cycle length of 500 </b><b>ms</b><b>. </b><b>Figure 10: </b><b>Comparison of the principal currents in the 0D and 1D computational models </b><b>under </b><b>steady-state pacing with a cycle length of 500 </b><b>ms</b><b>.</b><b>Figure 11: </b><b>Comparison of </b><b>experimental and computational </b><b>restitution behavio</b><b>u</b><b>r </b><b>of </b><b>several action potential (AP) </b><b>characteristics </b><b>for </b><b>the S1–S2 pacing protocol. </b><b>Figure 12: </b><b>Comparison of </b><b>experimental and computational </b><b>restitution behaviour </b><b>of </b><b>several action potential (AP) </b><b>characteristics for </b><b>the dynamic pacing protocol.</b><b>Figure 13: </b><b>Comparison of the experimental and computational restitution behaviour of </b><b>several</b><b> calcium transient </b><b>(</b><b>CaT</b><b>) </b><b>characteristics </b><b>for </b><b>the dynamic pacing protocol.</b><b>Figure 14: </b><b>Sensitivity analyses for the principal ionic current </b><b>conductances</b><b> and other key parameters </b><b>determining</b><b> characteristics of the action potential</b><b> (AP)</b><b> in the 0D and 1D computational models.</b><b>Figure 15: </b><b>C</b><b>ytosolic calcium (Ca</b>^{<strong>2+</strong>}<b>) flows contributing to the calcium transient (</b><b>CaT</b><b>) in the 1D computational </b><b>model</b><b>.</b>