Additional file 1 of Genome-wide identification and functional characterization of GPCR family genes reveal their key roles in the vitellarium development and egg production in Schistosoma japonicum

Additional file 1: Figure S1. S. japonicum GPCR classification and predicted upstream ligands. (a) Heatmap showing the transcript levels of GPCRs across different development stages. Class A included rhodopsin and adrenergic-like receptors (77 GPCRs), Class B included calcitonin and PTH-like receptors (3 GPCRs), Class C included Metabotropic-like receptors (1 GPCR), Class D included hormone-like receptors (5 GPCRs), and the unclassified (40 GPCRs) category included GPCRs with uncertain classification information. (b) Heatmap showing the transcript levels of predicted upstream ligands of GPCRs across different developmental points. They were categorized into different groups: amines (8 ligands), hormone proteins (1 ligands), lysosphingo lipids (4 ligands), nucleotide-like (2 ligands), olfactory (4 ligands), peptides (56 ligands), rhodopsin (2 ligands), and unclassified ligands (49 ligands). (c) Agarose gel electrophoresis analysis of PCR products to amplify the full-length CDS of eight selected GPCRs: EWB00_005311, EWB00_003955, EWB00_002138, EWB00_004787, EWB00_008255, EWB00_006379, EWB00_0011090 and EWB00_007147. M, DNA Marker.