Additional file 1 of Production of functional recombinant antibodies in Dictyostelium discoideum

Supplementary Material 1. Supplementary Fig. 1: Design of the expression vector prepSC3-scFv to produce recombinant antibodies in D. discoideum cells. a Schematic overview of the prepSC3 plasmid with the scFv fragment cloned in KpnI/BamHI. b Schematic of a minibody composed of a dimer of scFv-Fc fragments. (Signal P: signal peptide encoding sequence; VH and VL: variable domains of the heavy (VH) and light (VL) immunoglobulin chains; scFv: single-chain fragment variable; L1 and L2: peptide linkers; pA: D. discoideum polyadenylation and termination signal; neo: neomycin selection marker; ori: E. coli origin of replication; bla: beta-lactamase encoding gene.