Additional file 1 of Impact of temperature on vector competence of Culex pipiens molestus: implications for Usutu virus transmission in temperate regions

Additional file 1. Figure A1: Proportion of body and saliva positive samples at 22 ˚C and 20 ˚C. Saliva samples at 0 dpi were positive, likely from virus contamination in the mouthparts, and so here are forced to one (A). Body samples at 0 dpi were positive, likely from virus in the bloodmeal (B). Pools of mosquitoes were fed spiked blood containing USUV at a titre of 4 × 107 PFU/ml and were tested by real-time RT-PCR.