Additional file 1 of Endothelial Piezo1 stimulates angiogenesis to offer protection against intestinal ischemia–reperfusion injury in mice

Additional file 1. Figure S1. Validation of endothelial Piezo1 knockout mice.Gel electrophoresis of PCR endpoint products. The top panel demonstrated the presence of the Cre recombinase transgene, while the bottom panel confirmed the presence of LoxP sequenceswithin the Piezo1 gene.RTq-PCR analysis of Piezo1 abundance in murine intestinal endothelial cells from Piezo1fl/fl and Piezo1ΔEC mice.Representative WB images of Piezo1 in murine intestinal endothelial cells from Piezo1fl/fl and Piezo1ΔEC mice.Representative traces of intracellular Ca2+ changes (△) in murine intestinal endothelial cells from Piezo1fl/fl and Piezo1ΔEC mice in response to 5 µM Yoda1.Peak value of Ca2+ in murine intestinal endothelial cells from Piezo1fl/fl and Piezo1ΔEC mice in response to 5 μM Yoda1. Data are presented as mean ± SEM; ****P < 0.0001, compared with Piezo1fl/fl group. Figure S2. Validation of the angiogenic effect of Piezo1 in primary intestinal endothelial cells.: Immunofluorescence staining of VEGFR2 in primary intestinal endothelial cells. Scale bar, 100 µm.