Additional file 10 of γ-Secretase modulator resistance of an aggressive Alzheimer-causing presenilin mutant can be overcome in the heterozygous patient state by a set of advanced compounds

Supplementary Material 10: Figure S10. Ngn2-expressing iPSCs can be differentiated into cortical neurons after doxycycline induction. (A) Schematic workflow of the differentiation protocol (DOX, doxycycline; Puro, puromycine; 5FU, 5-fluorouracil), see Materials and methods for details. (B) Single channel and merged immunofluorescence stainings (DAPI (blue), Tau (green), MAP2 (pink) and Tuj1 (green)) of differentiated neurons at day 27. The merged picture is also shown in Fig. 5B. Magnification: 63x; Scalebar: 10 µm.