Additional file 1 of DNA 5mC methylation inhibits the formation of G-quadruplex structures in the genome

Additinal file 1: Fig. S1. Immunofluorescence staining of cancerous and noncancerous rectal tissues for the proliferation marker Ki-67. Fig. S2. The 5mC-modified sites in BmPOUM2 G4 (a), hPDGFR-β G4 (b) and hVEGF G4 (c). Fig. S3. qRT–PCR analyses of hPDGFR-β and hVEGF expression in 293 T cells (a, b) or SK-Hep-1 cells (c, d) treated with PDS or 5-aza-dC. Fig. S4. The repeatability of G4 CUT&tag experiments in wildtype 293 T or 293 T-DNMT1-KO cells. (a, c) IGV genome browser tracks of G4 CUT-tag replicates. The reads were aligned to human genome hg38 and normalized by bins per million. (b, d) Correlation plot between every two G4 CUT-tag biological replicates. Fig. S5. The repeatability of ATAC-seq experiments in wildtype 293 T or 293 T-DNMT1-KO cells. (a, c) IGV genome browser tracks of ATAC-seq replicates. (b, d) Correlation plot between every two ATAC-seq biological replicates. Fig. S6. The repeatability of WGBS experiments in wildtype 293 T or 293 T-DNMT1-KO cells. (a) Correlation heat map of WGBS replicates. (b) Circus plot showing genome-wide methylation rate, genes density and CG islands density. Fig. S7. The original images of the WB results shown in Fig. 2k. Fig. S8. The original images of the WB results shown in Fig. 2p. Fig. S9. The original images of the EMSA results shown in Fig. 4i, k, m. Table S1. The primers and oligonucleotide probes used in this study.