Additional file 2 of Celastrol improves endothelial function in diet-induced obesity mice via attenuating endoplasmic reticulum stress through the activation of AMPK pathway

Supplementary Material 2. Figure S2. Effect of celastrol on the expression of upstream regulator and downstream effectors of ER stress in diet-induced obesity (DIO) mice. DIO and control (Con) mice were treated with saline or celastrol (Cel, 100 μg/kg/day) intraperitoneally (i.p) for 8 weeks. (A-D) The protein expression of BiP, ATF4, XBP1, and CHOP in aortae was analyzed by western blotting. Data are expressed as the means ± S.E.M (n=6/group), *P <0.05, one-way ANOVA followed by Newman-keuls post hoc test.