<b><i>de novo</i></b><b> transcriptome assembly and annotation of </b><b><i>Gammarus pulex</i></b><b>: a valuable resource for ecotoxicogenomics</b>

This study presents the <i>de novo</i> transcriptome assembly and annotation of <i>Gammarus pulex</i>, a freshwater amphipod crucial to ecotoxicology due to its ecological significance and sensitivity to pollutants. Three distinct transcriptomes were generated from <i>G. pulex</i> collected at sites with varying levels of micropollutant exposure across Germany and Sweden. High-quality transcriptomes were assembled for each population (German, Swedish, and combined) using RNA sequencing (RNA-Seq). Functional annotation identified coding regions, signal peptides, transmembrane domains, and conserved protein domains within the transcripts. This comprehensive transcriptomic resource enhances our understanding of <i>G. pulex's</i> molecular responses to environmental stressors, particularly micropollutant stress. The identified genes and pathways provide potential biomarkers for ecological monitoring and facilitate future ecotoxicogenomic studies, further reinforcing <i>G. pulex</i> as a sentinel species for aquatic ecosystem health.Please refer to the "Readme.txt inside the directory to get an overview of the file structure and contents.<br>

Publication Details

Journal
Figshare
Published
2025-01-01
DOI
https://doi.org/10.6084/m9.figshare.28451624
Primary Topic
Environmental Toxicology and Ecotoxicology
Type
article
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article

de novo transcriptome assembly and annotation of Gammarus pulex: a valuable resource for ecotoxicogenomics

Figshare
Environmental Toxicology and Ecotoxicology
article

de novo transcriptome assembly and annotation of Gammarus pulex: a valuable resource for ecotoxicogenomics

article en

Abstract

This study presents the <i>de novo</i> transcriptome assembly and annotation of <i>Gammarus pulex</i>, a freshwater amphipod crucial to ecotoxicology due to its ecological significance and sensitivity to pollutants. Three distinct transcriptomes were generated from <i>G. pulex</i> collected at sites with varying levels of micropollutant exposure across Germany and Sweden. High-quality transcriptomes were assembled for each population (German, Swedish, and combined) using RNA sequencing (RNA-Seq). Functional annotation identified coding regions, signal peptides, transmembrane domains, and conserved protein domains within the transcripts. This comprehensive transcriptomic resource enhances our understanding of <i>G. pulex's</i> molecular responses to environmental stressors, particularly micropollutant stress. The identified genes and pathways provide potential biomarkers for ecological monitoring and facilitate future ecotoxicogenomic studies, further reinforcing <i>G. pulex</i> as a sentinel species for aquatic ecosystem health.Please refer to the "Readme.txt inside the directory to get an overview of the file structure and contents.<br>

Figshare
Life in Land
Openalex Percentile: Top 45%
Environmental Toxicology and Ecotoxicology
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<b><i>de novo</i></b><b> transcriptome assembly and annotation of </b><b><i>Gammarus pulex</i></b><b>: a valuable resource for ecotoxicogenomics</b> · Figshare (2025) | TGRS Research Map | TGRS