<b>Effects of N-Carbamylglutamate supplementation on cecal morphology, microbiota composition, and short-chain fatty acids contents of broiler breeder roosters</b>

The objective of this study was to assess the effects of N-Carbamylglutamate (NCG) supplementation on cecal morphology, microbiota composition, and short-chain fatty acids (SCFAs) contents in broiler breeder roosters. The results of the study demonstrated that NCG supplementation had positive effects on cecal morphology, microbiota composition, and SCFAs contents in broiler breeder roosters, while did not affect the body weight.And resulted in an increase in cecal muscularis thickness and villi epithelium thickness. Furthermore, NCG supplementation was found to have regulatory effects on the composition of cecal microbiota. Additionally, the study observed an increase in the content of butyric acid in the cecum of broiler breeder roosters fed with the NCG-containing diet compared to those fed with the basal diet. Spearman correlation analysis showed that the variation of cecal microbiota was closely related to the production of butyric acid as well as the improvement of muscularis and villi epithelium thickness in cecum. The increase of butyric acid content in cecum was positively correlated with the improvement of cecal muscularis and villi epithelium thickness. In conclusion, the findings of this study indicate that dietary supplementation of NCG in broiler breeder roosters can positively influence cecal morphology, microbiota composition, and butyric production.