Additional file 1 of Detection of Borrelia burgdorferi (s.l.) in Ixodes ricinus ticks collected in Iceland

Additional file 1. Table S1. Reaction volumes for the Borrelia spp. qPCR assay. Table S2. Reaction volumes for the Coxiella burnetii and Francisella tularensis. qPCR assays. Table S3. Reaction volumes for the Rickettsia spp. qPCR assay. Table S4. Reaction volumes for the TBEV. qPCR assay. Table S5. Reaction volumes for the Borrelia 5S-23S rRNA intergenic spacer assay. Table S6. Cycling conditions optimised locally and based on the conditions described in the TagMan Fast Universal PCR Mastermix Manual. Table S7. Details on the primers and probes used to detect target microorganisms. Table S8. Cycling conditions optimised locally and based on the conditions described by Drosten et al., 2002. PCR Kit: Superscript III Platinum One-step qRT-PCR kit. Table S9. Cycling conditions optimised locally and based on the conditions described in the Tag 2X Mastermix Manual. Table S10. Borrelia genotypes detected by sequencing the 5S-23S rRNA intergenic spacer.