Additional file 3 of Evodiamine induces ferroptosis in prostate cancer cells by inhibiting TRIM26-mediated stabilization of GPX4

Additional file 3. Fig. S3 Evodiamine treatments caused GPX4 protein instability by reducing TRIM26 expression. (A) PC3 and DU145 cells were treated with 1.0 μM evod for durations of 0, 6, 12, and 24 h. Following this treatment, the levels of GPX4 mRNA were analyzed using quantitative polymerase chain reaction (qPCR). (B-D) trim26 mRNA levels were analyzed by qPCR, and TRIM26 protein levels were examined by Western blot in control and trim26-overexpressing (trim26-oe) PC3 and DU145 cells.