Additional file 1 of The LysoPS/GPR174 axis drives metastatic progression in esophageal squamous cell carcinoma through cAMP-PKA-CREB signaling activation

Supplementary material 1: Fig. S1. Scoring criteria for immunohistochemical findings in TMA of ESCC. A Example of ESCC tumour tissue and adjacent normal epithelial tissue. B The percentage of positively stained cells was divided into five categories: <5% was 0, 6-25% was 1, 26-50% was 2, 51-75% was 3, and >75% was 4. C The intensity of immunohistochemical staining was divided into five categories according to conventional criteria: negative was 0; weak was 1; moderate was 2; or strong was 3. Fig. S2. Overexpression and knockdown of ESCC cell lines infected with GPR174 lentivirus. Table S1. Core sequences of lentivirus about shGPR174. Table S2. Primers used for GPR174 genes amplification.