Additional file 12 of Lettuce immune responses and apoplastic metabolite profile contribute to reduced internal leaf colonization by human bacterial pathogens

Supplementary Material 12: Fig. S6. Multidimensional scaling (MDS) (A) and principal component analysis (PCA) (B) plots representing the correlation among biological replicates used for the whole leaf RNA-sequencing and AWF metabolomic analyses, respectively. Leaves of the lettuce cultivars Green Towers, Lollo Rossa, and Red Tide were vacuum infiltrated with mock (sterile distilled water) or bacterial inoculum containing 5 x 105 CFU/mL of Escherichia coli O157:H7 or Salmonella enterica ser. Typhimurium 14028s. Leaves were sampled at 1- and 7-days post inoculation (DPI). Coordinates for MDS graphs were calculated using normalized read counts in the cmdscale function of the R software and the coordinates for PCA graphs were obtained by using normalized peak heights in the MetaboAnalyst 5.0 software.