Additional file 7 of Analysis of early childhood intestinal microbial dynamics in a continuous-flow bioreactor

Supplementary Material 7: Figure 6. Association of bacterial taxa and metabolites in replicate chemostat cultures. Correlation analysis of bacterial composition and metabolite abundances over time in three replicate chemostat cultures inoculated with the NS1 bacterial library. (a) Heatmap of correlation analysis of ASV composition and metabolite levels over time as measured by 1H NMR. Correlation coefficients were normalized using a z-score and illustrated by the color gradient. Metabolites and ASVs were ordered based on hierarchical cluster analysis within each dataset. (b, c) Scatter plot of variation over time of (b) Bacteroides stercoris or (c) Bacteroides uniformis relative abundances and acetate concentrations (mM) profiled by 1H NMR. Linear regression analysis and coefficients are shown.