CCR2+ MONOCYTE-DERIVED MACROPHAGES DRIVE CARDIAC HYPERTROPHY IN EARLY HFPEF

Supplemental material for the manuscript "CCR2+ MONOCYTE-DERIVED MACROPHAGES DRIVE CARDIAC HYPERTROPHY IN EARLY HFPEF" by Jana Raman et al. Supplemental Figure 1: M-mode echocardiography and total circulating monocytes in male mice exposed to HFpEF-associated comorbidities. Supplemental Figure 2: Female mice on the comorbidities model (ANGII + HFD + Salt) do not develop hyperlipidemia, obesity, hypertension or diastolic dysfunction. Supplemental Figure 3: Gating strategy and representative plots for identification of heart leukocytes. Supplemental Figure 4: Gating strategy and representative plots for identification of blood leukocytes. Supplemental Figure 5: CCR2-/- mice lack pro-inflammatory monocytes. Supplemental Figure 6: Protein expression of mitochondrial respiratory chain complexes remains unchanged. Supplemental Figure 7: Uncropped western blots of Mitofusin2 (MFN2), TOM20, and GAPDH. Supplemental Figure 8: Uncropped western blots of mitochondrial complex V, complex III, complex IV, complex II, complex I. Supplemental Table 1.