Phytochemical screening and green synthesis of silver nanoparticles from cinnamomum verum crude bark extract: evaluation of cell cytotoxicity and wound healing activity by in-vitro methods

Abstract Cinnamomum verum is widely cultivated in India for its culinary purposes. The Cinnamomum verum spice is ample in medicinally important primary and secondary metabolites because of its active molecules which plays a substantial role in various pharmacological activities. Thus, in this present study an attempt was made to check phyto synthesized nanoparticles and its potentiality against pharmacological activities. To asses this activity Silver nanoparticles were synthesized from Cinnamomum verum crude bark extract by phytomediated green synthesis method. In synthesis colour changes from yellow to reddish brown due to Plasmon resonance responses and it was observed during incubation period that indicates the synthesis of AgNPs. The reduction of pure Ag+ ions was monitored by measuring the UV-Vis spectrum at 540nm which exchibit sharp peak confirming the synthesis of AgNPs. The synthesized AgNPs were subjected to different physical characterization, which includes TEM, XRD and FTIR, analysis showed silver nanoparticles illustrates that they are in oval shape with less than average size of about 20- 100 nm. In XRD studies reveals that presence of crystalline nanoparticles average size about 40-50nm, and also FTIR spectrum analysis of the Synthesized AgNPs showed significant functional group with exhibited 8 intense bands at ~3323, ~2944, ~2832, ~1639, ~1449, ~1413, ~1114 and ~618 cm-1 respectively. The current study, focused on Cell cytotoxicity (MTT assay) and Invitro Wound healing property by scratch assay revealed that L929 cell lines were treated with 100μg/ml AgNPs for 24hrs. The result indicated that AgNPs induced the migration of L929 cells resulting in wound closure and results were compared with the standard-drug ascorbic acid as reference and results were observed under inverted microscopically. Further studies of this synthesized AgNPs of Cinnamomum verum regarding its bioactive compounds and their molecular mechanism may lead to formation of industrially important valuable drug in future days.