Fusarium weifangense S. L. Han, M. M. Wang & L. Cai

Fusarium weifangense S. L. Han, M. M. Wang & L. Cai, Studies in Mycology 104: 87–148. 2023. Fig. 4 Synonym. Fusarium caulendophyticum H. Zhang & Y. L. Jiang, Mycosphere 14 (1): 2092–2207. 2023. Description. Conidiophores arising from aerial mycelium, 14–18 μm long, unbranched or irregularly branched, often reduced to single phialides; aerial phialides monophialidic, subulate to subcylindrical, smooth- and thin-walled, with inconspicuous or absent periclinal thickening, 9.2–12.2 × 4.0–4.4 μm; aerial conidia hyaline, rarely ellipsoidal to falcate, slightly curved with almost parallel sides, tapering towards both ends, with a blunt to conical and slightly curved apical cell, blunt to barely notched basal cell, smooth and thin-walled, (1 –) 3–5 - septate; 1 - septate conidia: (14 –) 15–19 (– 20) × 3–4 μm (av. 17 × 3 μm, n = 8); 2 - septate conidia: (19 –) 19–21 (– 24) × 3–5 μm (av. 21 × 4 μm, n = 14); 3 - septate conidia: (22 –) 26–31 (– 34) × 3–6 μm (av. 28 × 4 μm, n = 22); 4 - septate conidia: (30 –) 35–36 (– 45) × 3–6 μm (av. 36 × 5 μm, n = 17); 5 - septate conidia: (31 –) 34–37 (– 46) × 4–6 μm (av. 38 × 5 μm, n = 15). Sporodochia salmon to orange, formed abundantly on surface of carnation leaves. Sporodochial conidiophores densely, bearing apical whorls of one phialide; sporodochial phialides monophialidic, subulate to subcylindrical, 16–24 × 2–3 μm, smooth. Sporodochial conidia falcate, slightly curved, tapering towards both ends, with a slightly elongated conical or whip-like curved apical cell, a foot-like to notched basal cell, (0 –) 4–5 - septate, hyaline, thin, and smooth-walled; 0 - septate conidia: 26–28 × 3–4 μm; 1 - septate conidia: (17 –) 26–36 (– 37) × 3–6 μm (av. 28 × 4 μm, n = 10); 2 - septate conidia: (20 –) 21–37 × 3–5 μm (av. 25 × 4 μm, n = 7); 3 - septate conidia: 21–33 (– 38) × 3–5 μm (av. 32 × 5 μm, n = 12); 4 - septate conidia: (31 –) 32–35 (– 44) × 3–6 μm (av. 36 × 4 μm, n = 22); 5 - septate conidia: (34 –) 40–45 (– 48) × 3–6 μm (av. 42 × 4 μm, n = 16). Chlamydospores not observed. Culture characteristics. Colonies on PDA incubated at 25 ° C in the dark, reaching 86–90 mm diameter in 7 d; surface white, flat, felty to velvety, aerial mycelia dense, colony margin entire; reverse white, odor absent. Colonies on OA incubated at 25 ° C in the dark, reaching 85–89 mm diameter in 7 d; surface white and aerial mycelia scant, radiate, reverse white, radiate, odor absent. Materials examined. China • Sichuan Province, Baoting Li and Miao Autonomous County, on leaves of Prunus salicina, 2 July 2023, Q. Y. Liu (HSAUP 20852, HSAUP 30852), living cultures SAUCC 5208 C-2 = CGMCC 3.27939, SAUCC 5208 C-3. Notes. Fusarium weifangense (LC 18333, ex-type strain) was proposed by Han et al. (2023). Fusarium caulendophyticum (CGMCC 3.25474, ex-type strain) was proposed by Zhang et al. (2023 a). Fusarium weifangense (LC 18333, ex-type strain) was the first to be discovered. Fusarium weifangense (LC 18333 and LC 18243) are clustered with Fusarium caulendophyticum (CGMCC 3.25474 and GUCC 191050.2) clade in the combined phylogenetic tree (Fig. 1, Suppl. material 4). Fusarium weifangense (LC 18333, ex-type strain) and Fusarium caulendophyticum (CGMCC 3.25474, ex-type strain) were similar in cal (0 / 535), rpb 2 (1 / 657), and tef 1 (2 / 462) sequences. We therefore considered the Fusarium caulendophyticum synonym of Fusarium weifangense. In this study, our strains (SAUCC 5208 C- 2 and SAUCC 5208 C- 3) are clustered with the Fusarium weifangense (LC 18333 and LC 18243) clade in the combined phylogenetic tree (Fig. 1). SAUCC 5208 C- 2 and SAUCC 5208 C- 3 were similar to the latter in cal (with 100 % sequence identity), rpb 2 (99.85 %), and tef 1 (98.70 %) sequences. Fusarium weifangense was previously isolated from wheat, Capsicum sp., Triticum sp., Medicago sativa, Lactuca sativa, Chenopodium quinoa, and Rosaceae roxburghii, and it has now been reported for the first time on Prunus salicina (Wang et al. 2019; Xia et al. 2019; Yin et al. 2021; Han et al. 2023; Zhang et al. 2023 a) (Suppl. material 3).