FORMULATION AND EVALUATION OF DICLOFENAC SODIUM PRONIOSOMES

Approaches are being adapted to achieve this goal, by paying considerable attention either to control the distribution of drug by incorporating it in a carrier system, or by altering the structure of the drug at the molecular level, or to control the input of the drug into the bioenvironment to ensure an appropriate profile of distribution. Different types of pharmaceutical carriers are present. They are – particulate, polymeric, macromolecular, and cellular carrier. Particulate type carriers also known as a colloidal carrier system, include lipid particles (low- and high-density lipoprotein-LDL and HDL, respectively), microspheres, nanoparticles, polymeric micelles and vesicular like liposomes, niosomes, pharmacosomes, virosomes, etc. Diclofenac sodium loaded proniosomes can be prepared by slurry method with Span 80 and cholesterol in the ratio of 1:1, 2:1, and 1:2. The powder is smooth surface both visually and microscopically. The main objective of the present study was to perform the drug entrapment study by aqueous drug determination method. There are two methods available to carry out drug entrapment studies. They are aqueous drug determination method and lysis method. The former method is involved in the estimation of unentrapped drug while the latter method is based on the drug entrapped in the vesicles. The latter method need the sample to be dialysed to remove unentrapped drug. Moreover, there may be some chance of hindrances in the spectroscopic determination of drug along with the lysing agent in the sample. Niosomal dispersions were obtained when different volumes (5,10 and 10 ml)were added to proniosome powder. High % drug entrapment was observed in the formulation PRNIO 3. Osmotic shock studies on vesicles showed that no significant change in the niosomal preparation was stored at normal saline. Key Words: LDL, HDL, Span 80, Proniosomes, Diclofenac sodium