Development and Validation of UV Spectrophotometric Method for the Determination of Aprepitant in Bulk and Capsule Formulation

Analytical method development and validation is critical in pharmaceutical discovery, development, and manufacturing. The current study discusses the creation and validation of a UV Spectrophotometric method for Aprepitant. Simple, accurate and cost efficient Spectrophotometric method has been developed for the estimation of Aprepitant in bulk and Capsule dosage form. The standard and sample solutions were produced by utilizing ethanol as solvent. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) was found at 264nm. Beers law was obeyed in the concentration range of 5-15μg/mL concentration. The line equation y = 43.2401x0.0013 with r2 of 0.998 was obtained. Linearity, accuracy, precision, LoD, and LoQ were validated in accordance with ICH guidelines. The proposed method could be used to analyse Aprepitant in bulk and capsule formulation for quality control purposes.