Pseudohumicola intercalarispora Sastoque, Stchigel & Cano 2025, sp. nov.

Pseudohumicola intercalarispora Sastoque, Stchigel & Cano, sp. nov. MycoBank MB 847821. Fig. 10. Etymology: From Latin intercalaris -, intercalary, and - spora, spore, due to the presence of holothallic intercalary conidia. Typus: Spain, Canary Islands, La Palma, Fuencaliente (Los Canarios), from soil of Teneguía volcano, 15 Jul. 2008, coll. M. Calduch & A.M. Stchigel, isol. A.P. Sastoque (holotype CBS H-25245, culture ex-type FMR 19235 = CBS 149962). On potato carrot agar after 2 wk at 25 °C: Mycelium composed of hyaline, septate, branched, smooth- and thin-walled, 1–2.5 μm wide hyphae, often forming spirals (Fig. 10E). Conidiophores undifferentiated. Conidiogenous cells integrated to the vegetative hypha. Conidia mostly holoblastic and 1-celled, solitary or less frequently in chains of two, sessile, on short conical denticles or on short side branches from the hyphae (Fig. 10E,F), smooth- and thick-walled, covered by mucilaginous and dark brown substance, becoming verrucose to reticulate when old (Fig. 10F), sometimes intercalary (holothallic) (Fig. 10F), then 1-celled, pale olivaceous brown to brown with dark brown walls, globose, subglobose, obovoid, pyriform, less frequently ovoid, obpyriform or ellipsoidal, (4–)7–9(–10) × (4–)7–10(– 11) μm (Fig. 10E, F). Acremonium-like synasexual morph and sexual morph not observed. Culture characteristics (after 7 d at 25 ° C): Colonies on PCA 20–30 mm diam., circular and flat; margin filamentous, regular to lobulated; surface showing white (6A1) aerial mycelium in the central area, surrounded by a brownish-grey (6F2) circle and with yellowish-white (4A2) narrow or wide margins; soluble pigment absent; reverse brownish-grey (6F2) to brown (6E5) with yellowish-white margins (4A2). Colonies on OA 29–33 mm diam., flat, circular, and expansive; margin filamentous and regular; surface with scarce and floccose grey (6E1) aerial hyphae only in the centre, bottom brownish grey (5F2) and margins bronze (5E5) without aerial hyphae; soluble pigment absent; reverse greyish brown (5F3) and margins hair brown (5E4). Colonies on CMA 22–23 mm diam., circular and flat; margin filamentous and regular; surface chocolate (6F4) with floccose greyish white (6B1) aerial hyphae in the central area, around and margins brown (5F6); soluble pigment absent; reverse chocolate (6F4) in the centre and wide tobacco (5F6) margins. Colonies on MEA 28–30 mm diam. after 7 d at 25 °C; flat and circular; margin entire and regular; surface brownish orange (6C3) with white (6A1) aerial hyphae in the centre, sand (4B3) and with yellowish-white (4A2) margins; soluble pigment absent; reverse pale orange (6A3) in the centre, surrounded by greyish-orange (5B3) and margins orange-white (5A2). Culture iconography after 2 wk of incubation on PCA, OA, CMA and MEA (Fig. 10A–D, respectively). Minimum, optimum and maximum temperature of growth on PDA after 7 d: 12 °C, 30 °C and 37 °C, respectively. Notes: Pseudohumicola intercalarispora was placed as a distinct species in a basal branch of a well-supported clade (1 PP/99 % BS) including Pseudohumicola alba COAD 3126 (Fig. 1). Pseudohumicola intercalarispora differs from P. alba by the presence of abundant intercalary conidia (absent in P. alba) and by the absence of an phialidic synasexual morph (present in P. alba) as well as the shape and size of humicolalike conidia [globose, subglobose, obovoid, pyriform, less frequently ovoid, obpyriform or ellipsoidal, (4–)7–9(–10) × (4–)7–10(–11) μm in P. intercalarispora and globose to subglobose or obovoid, with a truncated base, (8.3–)9.6–10.4(– 12.6) × (7.0–)8.8–9.6(–10.4) μm in P. alba] (Oliveira et al. 2023).