Sedative And Hypnotic Activity of Stenocereus Stellatus Extract on Chronic Unpredicatable Stress Induced Insomnia in Experimental Animal

Objective To investigate the effect of Stenocereus stellatus on the improvement of sleep quality in insomnia mice and its mechanism through a mice model of insomnia induced by chronic unpredictable stress. Methods A total of 30 swiss albino mice were randomly divided into 6 groups: control group, inducing group, positive drug group (diazepam 2.5 mg·kg-1 ), and high-, and low-dose groups 200 and 400 mg/kg , 6 in each group . swiss albino mice in other groups except the control group were stimulated by multiple factors for 14 days, to build a model of insomnia. After that, diazepam and three different doses of stomach soothing prescription were given for 14 days. Behavioral experiments and sodium pentobarbital-induced sleep experiments were performed separately. Results Compared with the CUS inducing group,. The Forced Swim Test (FST), a standard test for assessing depressive-like behavior, revealed increased immobility time in the stress control group, indicating behavioral despair. Mice treated with EESS showed a significant reduction in immobility time, suggesting antidepressant-like effects. Diazepam, used as a positive control, also significantly decreased immobility.In the Hole Cross Test, another measure of depressive behavior, the stress control group exhibited increased immobility. EESS-treated groups displayed improved activity levels, indicating a reduction in depressive symptoms. Diazepam again showed a strong positive effect.