A guide to selecting high-performing antibodies for Prosaposin (UniProt ID: P07602) for use in western blot and immunoprecipitation

This report provides a guide to selecting high-quality commercial antibodies against Prosaposin for use in Western blot and immunoprecipitation, utilizing a standardized experimental protocol that compares readouts in knockout cell lines and isogenic parental controls. This research was partly funded by the G-Can (GBA1-Canada) initiative, an open-science collaboration dedicated to advancing research on Parkinson's disease associated with GBA1 mutations. This work was also supported by the CQDM by a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec.