Diaporthe sennicola C. M. Tian & Qin Yang

Diaporthe sennicola C. M. Tian & Qin Yang, Phytotaxa 302 (2): 150 (2017) Fig. 11 Description. Pathogenic to okra (Abelmoschus esculentus) and causes brown stem lesion (canker) of the stem. Sexual morph not observed. Conidiomata pycnidial, immersed, scattered, circular, or ovoid in shape, with single, undivided loculus. Ectostromatic disc dark brown, flat, elliptical. Conidiophores hyaline, branched, phialidic, straight or partially curved, narrowing towards the apex, 11.5–17 × 1–1.6 μm (12.7 × 1.3 μm, n = 20). Conidiogenous cells hyaline, phialidic, straight, or partially curved. Alpha conidia hyaline, aseptate, elliptic, biguttulate, 5–9 × 1.3–2.7 μm (6.5 × 2.3 μm, n = 40). Beta and gamma conidia are absent. Culture characteristics. Colonies on PDA reach 50–55 mm in diameter after 7 days of growth at 25 ° C in the dark, flat, and white, turning into greyish rose with age. Compact, furcate mycelium is erratically distributed over the agar surface, edge irregular. Material examined. Thailand • Sakon Nakhon Province, Mueang Sakon Nakhon District, on stem lesion of okra (Abelmoschus esculentus), February 2023, Maryam Fallahi, dried culture MF 148-2 (MFLU 24-0243), living culture, MFLUCC 24-0241. Notes. In the phylogenetic tree generated in this study, strain MFLUCC 24-0241 clustered with strains of Diaporthe sennicola (CFCC 51634 (ex-type) and CFCC 51635) with 100 % ML bootstrap support, and 1.0 BYPP (Fig. 10). The base pair differences between D. sennicola strains MFLUCC 24-0241 and ex-type CFCC 51634 revealed 2.4 % (11 / 455 bp) differences in ITS, 2.9 % (8 / 276 bp) differences in tef 1, 2.8 % (13 / 464 bp) differences in tub 2, and 0.4 % (2 / 455 bp) differences in cal. The sequence data of his 3 is not available for D. sennicola (CFCC 51634). Diaporthe sennicola (MFLUCC 24-0241) is similar to D. sennicola (BJFC-S 1368, holotype) (Yang et al. 2017). Based on phylogenetic analyses conducted by Norphanphoun et al. (2022), it was clustered in the D. alnea species complex. In the phylogenetic tree of Dong et al. (2020), D. sennicola is similar to D. clausenae. However, D. clausenae can be distinguished from D. sennicola by the presence of 2–4 large or small guttules in alpha conidia, as well as the presence of beta conidia (Hongsanan et al. 2023). This study identifies okra as a new host for D. sennicola.