Validated Uv Spectrophotometric Quantification of Dutasteride In Pharmaceutical Formulations by Using Multivariate Technique

<p><span>The Present work focuses an accurate, simple and precise UV method with multivariate calibration technique for the estimation of Dutasteride in pharmaceutical dosage form. Multivariate calibration method uses the linear regression equations by correlating the relation between concentration and absorbance at 5 different wavelengths. The λmaxof Dutasteride shows at 269 nm and obeyed Beers law in the range of 1-5μg/ml. The percentage recovery of tablet formulation was found to be in the range of 98.50 to 99.88%. The limit of quantification and limit of detection were found to be 1.272 and 0.0420µg/ml respectively. The low percentage RSD values are indicated the accuracy and precise of the method. The present method can be successfully applied for method development and validation by multivariate analysis of Dutasteride.</span></p>