Evaluation of total phenolic and flavonoid content and antioxidant activity of Ophiorrhiza ochroleuca Hook.f.

The present study was carried out to evaluate total phenolic content, total flavonoid content, and antioxidant activity from methanolic extracts of the stem and leaves of Ophiorrhiza ochroleuca Hook.f. (Rubiaceae). The total phenolic content of both leaf and young stem was determined using the Folin-ciocalteu reagent method. Leaf displayed the highest phenolic content (43.97 ± 0.71 mg gallic acid equivalent (GAE)/g of dry sample extract followed by the stem with 16.24 ± 0.76 mg GAE/g). while the highest flavonoid content was observed in leaf extract with the result 90.40 ± 1.20 mg rutin equivalent (RE)/g of dry sample. The antioxidant activity was determined using DPPH (2,2-dyphenyl-1-picrylhydrazyl) assay with ascorbic acid as standard (IC50 value 6.65 ± 0.22). Leaf reported the highest antioxidant activity with IC50 value 508.81 ± 17.60 μg/ml followed by the stem with IC50 value of 581.62 ± 45.22 μg/ml.