A Comprehensive Review on the Method Development and Analytical Estimation of Metformin and Gliclazide

A simple rapid, accurate, precise, specific and economical spectrometric method for simultaneous estimation of Gliclazide (GLC) and Metformin hydrochloride (MET) is combined tablet dosage form has been developed. First method is based on an equation of area calculation of curve at two wavelength regions (228.6 to 224nm and 235 to 231 nm). Second method employs simultaneous equation at two wave length corresponding to 226.3 and 233.2 nm. Third method employs derivative spectroscopy at zero crossing points (226 and 232.9) in first order derivative spectra. Both the drugs obey Beer’s law in the concentration ranges employed for these methods.