Torulaspora incommunis M. Silva, F. Paraiso, M. Groenewald & J. P. Sampaio 2025

Torulaspora incommunis M. Silva, F. Paraíso, M. Groenewald & J.P. Sampaio, sp. nov. MB 853859. Fig. 4K, L. Etymology: Torulaspora incommunis. in.com’mu.nis, L. fem. adj. incommunis, uncommon, referring to the apparent rarity of this species that, albeit first isolated more than 90 years ago, is known from a very low number of isolates. Typus: Japan, Wakayama, sap of an orange tree (Citrus sp.), 1932, unknown collector (holotype PYCC 9318H, ex-holotype cultures PYCC 9318, CBS 5080, IFO 0022). The holotype is permanently maintained in a metabolically inactive state in the Portuguese Yeast Culture Collection, Caparica, Portugal. The genome of this species was deposited at DDBJ /ENA/GenBank under the accession GCA_012851155. The version described in this paper is v. 1. Description: After 1 wk on YM agar at 25 °C, cultures are smooth, cream-coloured, and butyrous. After 3 d of growth on YM agar at 25 °C, cells are ellipsoidal (4–5 × 2.5–4 µm) and occur singly or in pairs, and proliferation is by multilateral budding (Fig. 4K). On Dalmau plates after 2 wk at 25 °C, no pseudohyphae nor true hyphae are formed. Sexual reproduction is observed on acetate agar after 20 d at 20 °C, and the cultures appear to be homothallic. Asci are persistent and are formed after cell-to-cell conjugation frequently involving a cell and its bud and occasionally two independent cells. Asci produce one to two smooth and spherical ascospores, measuring 2.5–3 µm diam. (Fig. 4L). The physiological and biochemical profile of the species in shown in Table 1 and Table S5. Habitat and distribution: This species was found in the sap of an orange tree (Citrus sp.), Wakayama, Japan. Two additional cultures tentatively identified as this species were found in a rose flower in Fukuyama, Hiroshima, Japan and in soil in Taiwan. Additional cultures examined: During the investigation of D1/ D2 and ITS sequences deposited at GenBank, two additional representatives of this species were presumptively detected (Table S2, Figs S2, S 3): SG5S08, found in soil, Taiwan and FR 994 (NBRCN 114950), found in a rose flower, Fukuyama, Hiroshima, Japan (Hisatomi & Toyomura 2021).