Saturated salt solution (SSS) embalming: an alternative approach

Embalming is a crucial practice in veterinary anatomy for the preservation of animal cadavers, allowing extended study and dissection while preventing decomposition. Traditional embalming methods have relied heavily on formalin, a formaldehyde-based fixative known for its tissue preservation capabilities but also for its adverse health effects, including respiratory irritation and carcinogenicity. This study explores an alternative embalming technique using a saturated salt solution (SSS) as a non-toxic, cost-effective, and accessible fixative. The proposed method involves a sequence of vascular perfusion steps: initial flushing of blood vessels with a 0.9% saline solution, followed by continuous perfusion and recirculation of SSS over 6–8 hours. This technique avoids the need for refrigeration, maintains tissue pliability and natural consistency, and significantly reduces health risks to handlers. Moreover, the effectiveness of preservation varies with the route of vascular access, with deeper cannulation offering superior results for visceral organs. This method offers a promising alternative for veterinary anatomy teaching and research, promoting a safer and more sustainable approach to cadaver preservation.