Development And Validation of RP-HPLC Method for Estimation of Esmolol HCL from Semisolid Dosage Form

Esmolol Hydrochloride belongs to the class II anti-arrhythmic drugs. Topical Esmolol Hydrochloride used as a Novel Treatment Modality for Diabetic Foot Ulcers. Development and validation of Simple, Precise and Accurate RP-HPLC method for estimation of Esmolol Hydrochloride in Semi solid dosage form. The validation of this method was achieved as per ICH Q2 (R2) guidelines with the optimized experimental conditions. To achieve the proposed method on Intsil C18 column (300 mm x 3.9 mm, 5 µm) column as Stationary Phase and run time was 20 min. The Mobile Phase consists of Acetonitrile:6.8g/L Ammonium acetate buffer (15:85) (pH 5). UV detection was carried out at 221nm. Linearity co-relation co-efficient found is 0.99965. The method was validated by determining its accuracy, linearity and precision. The proposed method is simple, precise, economical and hence can be applied for routine quality control of esmolol hydrochloride in semi solid dosage form.