Kockovaella sambucuse C. Y. Chai & F. L. Hui 2024, sp. nov.

Kockovaella sambucuse C. Y. Chai & F. L. Hui sp. nov. Fig. 5 Etymology. The specific epithet sambucuse refers to Sambucus, the plant genus from which the type strain was isolated. Type. China • Guizhou Prov.: Guiyang City, Guiyang Botanical Garden, in the phylloplane of Sambucus chinensis, Aug 2022, L. Zhang and F. L. Hui, NYNU 22942 (holotype GDMCC 2.313 T preserved as a metabolically inactive state, culture ex-type PYCC 9951). Description. On YM agar after 7 days at 20 ° C, the streak culture is white to cream-colored, butyrous, smooth and glistening, with an entire margin. After 7 days in YM broth at 20 ° C, cells are ovoid, 2.1–3.3 × 3.3–4.7 μm, and single or pairs, budding is polar. After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar. Ballistoconidia are ellipsoidal or somewhat kidney-shaped, 3.4–4.9 × 5.2–6.8 μm. Glucose fermentation is absent. Glucose, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, cellobiose, salicin, L-sorbose (delayed), L-rhamnose (delayed), D-xylose, L-arabinose, D-arabinose, D-ribose, glycerol (delayed), ribitol, galactitol, D-mannitol, D-glucitol, DL-lactate (delayed and weak), succinate, citrate, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-D-gluconate (weak), D-glucuronate and glucono- 1.5 - lactone are assimilated as sole carbon sources. Inulin, methyl-α-D-glucoside, 5 - keto-D-gluconate, methanol, ethanol, erythritol, myo-inositol, and D-gluconate are not assimilated. L-Lysine is assimilated as sole nitrogen sources. Nitrate, nitrite, ethylamine and cadaverine are not assimilated. Maximum growth temperature is 30 ° C. Growth in vitamin-free medium is positive. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive. GenBank accession numbers. holotype GDMCC 2.313 T (ITS: OP 566879, D 1 / D 2: OP 566878). Note. Physiologically, Kockovaella sambucuse sp. nov. differs from three closely related species, K. haikouensis, K. ischaemi, and K. libkindii, in its ability to assimilate L-sorbose and its inability to assimilate cadaverine (Table 3).