Preclinical in-vitro antihyperglycemic potential of fresh leaf juice of Coleus amboinicus (L.)

High postprandial glucose must be controlled to prevent problems, and high plasmaglucose is caused by α-amylase and α-glucosidases. In modern clinical practice, enzymeinhibitors such as acarbose, voglibose, and others are recognised to have a number ofgastrointestinal adverse effects. In order to circumvent the toxicity and side effects, thecurrent work sought to identify potential natural α-amylase and α-glucosidase inhibitorsusing in-vitro antidiabetic assays. When tested against fungal α-amylase and α-glucosidases isolated from the small intestine of albino rats, different concentrations ofColeus amboinicus Lour. leaf juice (25, 50, 75, 100, and 125 µg/ml) showed a noticeabledose-dependent inhibition of the enzymes that was comparable to that of the commercialproduct, Acarbose. LJCA and acarbose were shown to have IC50 values of 82.15 and51.15 µg/ml on fungal α-amylase, respectively. LJCA and acar-bose were shown to haveIC50 values of 91.44 and 53.84 µg/ml on α-glucosidase, respectively. Leaf juice wasdiscovered to have a protein content of 10.6 mg/ml.