Isolation and Evaluation of Anti-Prevotella Activity of Moringa Oleifera Leaf Extract by HPLC

This study aimed to isolate and evaluate the antimicrobial and anti-biofilm activity of Moringaoleifera leaf extract against Prevotella species, with identification and quantification of bioactive compounds using High-Performance Liquid Chromatography (HPLC). Materials and Methods: Fresh Moringaoleifera leaves were shade-dried, powdered, and extracted with ethanol using the Soxhlet method. Phytochemical screening was performed to detect alkaloids, flavonoids, tannins, saponins, and phenolic compounds. HPLC analysis at 280 nm and 320 nm was conducted to quantify major phytochemicals. Prevotella species were isolated from clinical dental plaque samples and confirmed through Gram staining, anaerobic culturing, and biochemical characterization. Antimicrobial activity of the extract was assessed using agar well diffusion, while Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using broth microdilution. Anti-biofilm activity was evaluated using a crystal violet staining assay in 96-well microtiter plates. Results: HPLC analysis revealed significant levels of phenolic acids (e.g., chlorogenic acid, ferulic acid) and flavonoids (e.g., quercetin, rutin). The ethanolic extract exhibited strong antimicrobial activity, with a zone of inhibition of 18.4 ± 1.1 mm, MIC of 62.5 µg/mL, and MBC of 125 µg/ml against Prevotella spp. The extract also demonstrated 68.3% inhibition of biofilm formation at the MIC level. Conclusion: Moringaoleifera leaf extract showed potent anti-Prevotella and anti-biofilm activities, suggesting its potential as a natural therapeutic agent for oral infections.