Optimizing HPLC Protocols for Accurate Analysis of Sulopenem Etzadroxil and Probenecid: A Systematic Review

Sulopenem etzadroxil is a prodrug of the penem antibacterial sulopenem. Like other beta-lactam antibacterials, it is a time-dependent inhibitor of bacterial cell wall synthesis. Probenecid is a medication used to treat gouty arthritis, tophaceous gout, and hyperuricemia. The FDA approved sulopenem etzadroxil and probenecid (Orlynvah; Iterum Therapeutics) for the treatment of uncomplicated urinary tract infections (uUTIs) caused by Escherichia coli, Klebsiella pneumoniae, or Proteus mirabilis in adult women with limited or no alternative oral antibacterial option. HPLC is the dominant separation technique to detect, separate and quantify the drug. A number of chromatographic parameters were analyzed to optimize the method like sample pretreatment, choosing mobile phase, column, and detector selection. . Method development is a broad term. In both quantitative and qualitative analysis developing a new method either for estimation of quantity of substance or to check the presence of the required component is a necessitate