Development of Green Silver Nanoparticles With Wound Healing Property

Silver nanoparticles (AgNPs) are recognized for their broad-spectrum antimicrobial properties. This study focuses on the green synthesis of AgNPs using Catharanthus roseus leaf extracts, which eliminates the need for toxic chemicals and energy-intensive methods typically associated with conventional nanoparticle synthesis. The objective is to produce AgNPs with antimicrobial activity specifically targeting skin pathogens. Aqueous extracts from the leaves of the eco-friendly herb C. roseus were employed in the synthesis process. Phytochemical analysis of these extracts revealed the presence of alkaloids, phenols, saponins, and quinine. The successful synthesis of AgNPs was confirmed via UV-visible spectroscopy. The AgNPs synthesized using C. roseus extracts were evaluated for their antioxidant and antimicrobial activities against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The nanoparticles demonstrated significant antioxidant and antimicrobial effects. Additionally, the wound healing potential of these AgNPs was assessed through the inhibition of protein denaturation assay, which indicated promising wound healing capabilities. These findings highlight the potential of green-synthesized AgNPs for the development of wound healing formulations.