In-vitro antioxidant activity of hydroalcoholic extract of leaves of Hydnocarpus pentandrus (Buch. - Ham) Oken

Cellular damage can arise from the reactions of free radicals with membrane lipids, nucleic acids, proteins, enzymes, and other micro molecules. Free radical-induced cell damage seems to be a primary factor in the aging process and degenerative diseases, including but not limited to cancer, heart disease, cataracts, liver disorders, diabetes mellitus, inflammation, and renal failure. Naturally, the body produces free radicals, and antioxidants scavenge them to shield the body from harmful consequences. This dynamic equilibrium exists between the two. It's possible that there aren't enough antioxidants in the body under typical physiological circumstances to offset the production of free radicals. Thus, it stands to reason that adding antioxidants to our diet will help shield us from dangerous illnesses. Thus, the creation of "natural antioxidants" from plant material has drawn more attention from the food business and preventative medicine. Given the importance of antioxidant activity, a hydroalcoholic crude extract from the leaves of the Achariaceae family plant Hydnocarpus pentandrus (Buch. - Ham) Oken was made, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) Radical Scavenging Assay, the Ferric Ion Reducing Assay (FRAP), the Nitric Oxide Assay, and the H2O2 Radical Scavenging Assay were used to measure the radical scavenging activity. The antioxidant activity of the hydroalcoholic extract was examined in comparison to the reference; in all four methods, solvent-based findings were observed. Higher antioxidant activity is shown by the FRAP and DPPH assays, which are followed by the nitric oxide, and H2O2 radical assays. DPPH > FRAP > H2O2 > Nitric oxide.