A Novel Validated Uplc Method for The Determination of Prucalopride Succinate in Solid Dosage Form

The objective of the current study was to develop a novel technique, simple, precise, rapid, reproducible and selective UPLC method for the estimation of Prucalopride Succinate in bulk and tablet dosage form. Estimation was achieved by C18 (50mm×3mm×1.7µ) column using Potassium dihydrogen ortho phosphate and Methanol, Ratio 60:40 %w/w at ambient temperature. Flow Rate was 0.8ml/min, injection volume 2 µl and detectable wavelength at 225nm. The retention time for Prucalopride succinate was 0.61 minutes. The method was validated for specificity, linearity, precision, accuracy; robustness .The linearity of the proposed method was at the range of 50-150µg/ ml of Prucalopride succinate with a correlation coefficient of 0.9999. The precision (relative standard deviation – RSD) of six samples was 0.5066%. The accuracy (recovery) was 100.07 % and 100.19 %.and 99.93%.