The Identification of Local Upland Rice from Central Sulawesi Using PCR Technique and Chemical Properties

Abstract: Restu Dwi Nugroho, Rostiati Dg Rahmatu, Nur Edy (Identification of Local Upland Rice from Central Sulawesi Using PCR Technique and Physicochemical Properties. (Supervised by Rostiati, Nur Edy) Rice is a staple food in Indonesia and one of the crops that provides 20% of the total food calories globally. National rice production has so far been focused on irrigated rice fields, especially on the island of Java, while the contribution of upland or rainfed rice, which is scattered across various islands in Indonesia, remains very limited. This could pose a problem in the future due to the rapid population growth, increased food demand, especially for rice, and the reduction of rice fields, which have been the primary land for rice production. Although national production of upland rice is relatively small compared to irrigated rice, upland rice has great potential to be developed, particularly in drylands. Efforts to promote the development of upland rice in Central Sulawesi include optimizing high-quality planting materials or seeds. One approach to identifying the diversity of upland rice in Central Sulawesi is through the identification of its physicochemical and genetic properties. Physicochemical analysis was carried out to assess antioxidants, moisture content, fat content, amylose, amylopectin, carbohydrate content, protein content, and fiber content. Genetic identification was performed to determine genetic diversity. Genetic diversity identification was conducted using PCR sequencing. The results of the study showed that the phytochemical analysis through various tests demonstrated superior chemical properties. In the DNA analysis, DNA amplification was conducted to observe DNA bands using the rbcLa-F and Rbcl-724R primers, which produced distinct bands in the five local upland rice cultivars from Central Sulawesi, yielding amplicons ranging in size from 100 to 1000bp. Keywords: Genetic Diversity, Physicochemical, PCR, Local Upland Rice, Sequencing. Title: The Identification of Local Upland Rice from Central Sulawesi Using PCR Technique and Chemical Properties Author: Restu Dwi Nugroho, Rostiati Dg Rahmatu, Nur Edy International Journal of Life Sciences Research ISSN 2348-313X (Print), ISSN 2348-3148 (online) Vol. 12, Issue 4, October 2024 - December 2024 Page No: 30-36 Research Publish Journals Website: www.researchpublish.com Published Date: 21-November-2024 DOI: https://doi.org/10.5281/zenodo.14199232 Paper Download Link (Source) https://www.researchpublish.com/papers/the-identification-of-local-upland-rice-from-central-sulawesi-using-pcr-technique-and-chemical-properties