Assessment of cellulosic supports for one-step purification/immobilization of CBM-tagged HMF oxidase

The cost of the biocatalyst is one of the main bottlenecks in the industrial implementation of enzymes. The use of strategies such as one-step purificationand immobilization can reduce the costs of the downstream process, which contributes 50-80% of the total production cost (1). Furthermore, enzymeimmobilization allows to easily recovery and re-used, or applied in continuous processes allowing process intensification while their stability towardschemo-physical process conditions is improved.New technologies of immobilization are emerging based on biomolecular scaffolds, such as cellulose binding modules (CBMs), that could be geneticallyfused to enzymes to allow specific adsorption into cellulosic materials without the need of chemical functionalization (Fig. 1), allowing a more cost-effective and eco-friendly strategy of immobilization (2).In the current work, the assessment of different commercial cellulosic supports for the one-step purification/immobilization of a 5-Hydroxymethylfurfuraloxidase (HMFO) and its applications in the synthesis of a renewable building block, 2,5-Furandicarboxylic acid (FDCA), was carried out.