Formulation of Antimicrobial gel of Adhatoda vasica Leaf Extract

The present study investigates the antimicrobial activity of Adhatoda vasica leaves extract and its potential application in developing a topical antimicrobial gel. The study began with the preparation of ethanolic and aqueous extracts of Adhatoda vasica leaves, followed by phytochemical screening to identify bioactive compounds. Antimicrobial activity was evaluated against pathogenic microorganisms, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans, using the agar well diffusion method. The results revealed significant antimicrobial activity, particularly in the ethanolic extract, attributed to alkaloids, flavonoids, and phenolic compounds. Based on these findings, a topical gel formulation was developed using the ethanolic extract, and its physicochemical properties, stability, and antimicrobial efficacy were evaluated. The gel exhibited promising antimicrobial properties, good spreadability, and stability under various conditions. This study highlights the potential of Adhatoda vasica as a natural source for antimicrobial agents and provides a basis for the development of herbal topical formulations for combating microbial infections.