Physicochemical Standardization And Evaluation Of Immunomodulatory Activity By In-Vitro Method Of Centella Asiatica Linn. Urban

Centella Asiatica is the most known medicinal herb in the Indian sub-continent. It is commonly known as Gotu, Kola, Mandukparni, and Bramhi. It has been used since ancient Ayurveda due to its being rich in phytochemicals like Madecassoside, Asiaticoside, Asiatic acid, Bramhic acid, D-mannitol, and centellose, etc. These triterpenoids and trisaccharides make the plant medicinal. About 124 chemical compounds are present in Centella Asiatica. These phytocompounds enhance the immunomodulatory potential. Bramhi is being used widely as a powerful antioxidant, helps during anxiety, boosts immunity, and improves cognitive functions. A recent study has proved that due to its phytochemicals like flavonoids present in it, it has very effective immunomodulatory activity. These studies are supported by some experiments carried out. So in our study, we have performed in-vitro studies which have shown some promising results by having cell proliferation and low cytotoxicity. We have performed an in-vitro study (MTT assay) by using Umbilical cord Mononuclear cells (UC-MNCs) which has critical components of the immunity system and they play a vital role in cell-mediated immunity. Elemental analysis was carried out to check the composition of elements present in it. As per the study, we have understood that it has stable immunomodulatory properties with low cytotoxicity and it would be the best alternative for immune disorders.