Evaluation of antimicrobial activity of plant extracts and column fractions of hexane extract against Mycobacterium kansaii

The aim of this study is to assess the antibacterial efficacy of aerial parts of the Ageratum conyzoides plant extracts and column fractions against Mycobacterium kansaii. The antimicrobial experiments were performed using five doses (0.1 µg/ml, 1 µg/ml, 10 µg/ml, 100 µg/ml, 1000 µg/ml) of plant extracts and column fractions. The results showed notable inhibitory effects on Mycobacterium kansaii. The results indicated that the extract of A. conyzoides exhibited action against all the tested bacterial isolates, with the level of activity varying depending on the concentration. The hexane extract exhibited antibacterial activity, with mean zones of inhibition measuring 97.88±9.46 and 27.1±3.33, while the ethanol extract showed a mean zone of inhibition of 100.6±3.42 and 36.77±1.74 against M.kansaii, respectively. Nevertheless, the hexane extract's three column fractions exhibited average inhibition zones of 98.60±1.0 and 23.11±4.35, 90.02±10.22 and 21.94±6.77, 99.32±3.04 and 17.23±5.26 for the third, fourth, and fifth (last sample) respectively. The findings of this study provide evidence for the conventional utilization of the Ageratum plant and emphasize the necessity for further comprehensive research to explore potential alternatives to current antibacterial medications.