High-Resolution Mass Spectroscopy Study of Smriti Panchak Yoga with An Emphasis on Antioxidant and Neuroprotective Effect

Ayurveda includes various medicinal herbs that are responsible for the development of the brain and neuroprotective action of the brain. In accordance, with that we have designed a new formulation named Smriti Panchak Yog (SPY) having 5 herbal extracts i.e. Brahmi, Shankhpushpi, Vacha, Tagar, and Ashwagandha. Ensuring the quality, safety and efficacy of herbal drugs through standardization is crucial in advance scientific understanding and promote their clinical use. Procurement and authentication of raw drugs and preparation of hydroalcoholic extract of each drug separately by Soxhlet method of extraction. An equal quantity of dried and solvent-free extract was mixed and stored as Smriti Panchak Yog (SPY). High-Resolution Mass Spectrometry System (HRMS). The plant extract showed antioxidant activity through radical scavenging activity. The analysis of the phytoconstituent revealed that SPY has antioxidant capacity refers to the ability of a substance to neutralize or prevent the harmful effect of reactive oxygen species. DPPH and total phenolic content study revealed that SPY has high antioxidant capacity and neuroprotective effect reflecting the better neuropharmacological activity of SPY like 4-(trifluoromethyl)-1H-imidazole enhancement of memory, Choline ameliorate memory impairment. Valpromide shows antiepileptic and antipsychotic activity, Betaine plays a Neuroprotective role etc. The present study reveals that it has many bioactive compounds that reduce oxidative stress, increase memory and coordination power and also regulate inflammation and apoptosis. This is due to the presence of high phenolic content resulting in high antioxidant activity and neuroprotective power.