Supplementation of Coenzyme Ubiquinone (COQ10) in the semen diluent of Balitbangtan's Superior Native Chicken in Terms of Spermatozoa Cromatin Damage

Abstract : The development of technology in the field of animal husbandry is growing very rapidly, apart from ruminants from poultry livestock can also be done artificial insemination used for breeding. According to Apriyanti (2017) The success of the mating system can be seen from the success of livestock mating which is influenced by the quality of the semen produced. One of the supplementations used is coenzyme ubiquinone (CoQ10) where CoQ10 is one of the vitamins such as fat-soluble vitamin E which is endogenously present in the inner membrane of mitochondria of mammals and plants (El-Sayed, et al. 2021). This study used an experimental method or field experiment with 4 treatments and 10 replicates on 4 male KUB chickens aged 12 months and the diluent used was egg yolk lactated ringer. The variables observed in this study wes cromatin damage of spermatozoa before freezing and post thawing. The results obtained in this study chromatin damage before freezing obtained the highest average value of 1.18 ± 0.58 with statistical calculations showed significantly different results (P < 0.05). chromatin damage after thawing back obtained the highest result of 2.80 ± 0.89 with statistical test calculations obtained results that were not significantly different (P < 0.01) to the different levels of Coenzyme ubiquinone in diluents with different levels.