A guide to selecting high-performing antibodies for Rab3A (UniProt ID: P20336) for use in western blot, immunoprecipitation, and immunofluorescence

This article presents characterization data that can be utilized as a guide to select high-performing antibodies for Rab3A, a member of the Rab GTPase family of proteins involved in vesicle trafficking. Sixteen antibodies were evaluated by western blot, immunoprecipitation and immunofluorescence experiments using a standardized protocol that compares readouts in isogenic parental controls and knockout cell lines. Thank you to the Emory-Sage-SGC TREAT-AD center established by the National Institutes of Aging for supporting this study (grant number U54AG065187).

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2024-09-04
DOI
https://doi.org/10.5281/zenodo.13685052
Primary Topic
Cellular transport and secretion
Type
article
Field-Weighted Citation Impact
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article

A guide to selecting high-performing antibodies for Rab3A (UniProt ID: P20336) for use in western blot, immunoprecipitation, and immunofluorescence

Zenodo (CERN European Organization for Nuclear Research)
Cellular transport and secretion
article

A guide to selecting high-performing antibodies for Rab3A (UniProt ID: P20336) for use in western blot, immunoprecipitation, and immunofluorescence

article en

Abstract

This article presents characterization data that can be utilized as a guide to select high-performing antibodies for Rab3A, a member of the Rab GTPase family of proteins involved in vesicle trafficking. Sixteen antibodies were evaluated by western blot, immunoprecipitation and immunofluorescence experiments using a standardized protocol that compares readouts in isogenic parental controls and knockout cell lines. Thank you to the Emory-Sage-SGC TREAT-AD center established by the National Institutes of Aging for supporting this study (grant number U54AG065187).

Zenodo (CERN European Organization for Nuclear Research)
Montreal Neurological Institute and Hospital (CA), McGill University (CA)
Openalex Percentile: Top 56%
Cellular transport and secretion
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