Kazachstania paragamospora C. Y. Chai & F. L. Hui 2024, sp. nov.

Kazachstania paragamospora C. Y. Chai & F. L. Hui sp. nov. Fig. 2 C Etymology. the specific epithet paragamospora, like gamospora, referring to its phylogenetic closeness to Kazachstania gamospora. Type. Mexico • State of Mexico, Teotihuacan, in the traditional Mexican alcoholic beverage Pulque, autumn of 2015, F. L. Hui, NYNU 161111 (holotype CICC 33274 T preserved in a metabolically inactive state, culture ex-type CBS 15233). Description. After 3 days culture in YM broth at 25 ° C, the cells are spherical or ovoid (3–6.2 × 3.3–7.5 μm) and occurred singly or in pairs (Fig. 2 C). Budding is multilateral. Sediment is formed after one month, but no pellicle is observed. After 3 days cultured on YM agar at 25 ° C, the streak culture is butyrous, white, raised with a smooth surface, and has a complete margin. Asci or signs of conjugation are not observed on sporulation media. Glucose, sucrose, raffinose, and inulin are fermented, while trehalose, galactose, maltose, melibiose, lactose, cellobiose, melezitose, methyl α - D-glucoside, soluble starch, or xylose are not. Glucose, sucrose (weakly), raffinose (weakly), inulin, glycerol (weakly), L-arabinitol (weakly), 5 - keto-D-gluconate (weakly), D-gluconate, D-glucosamine (weakly), succinate (weakly), methanol (weakly) and soluble starch (weakly) are assimilated. No growth occurred in the presence of melibiose, galactose, lactose, L-sorbose, L-rhamnose, L-arabinose, D-arabinose, D-ribose, ethanol, erythritol, galactitol, myo - inositol, DL-lactate, xylitol, D-glucuronate, D-glucono- 1, 5 - lactone, trehalose, maltose, melezitose, methyl α-D-glucoside, cellobiose, salicin, D-xylose, ribitol, mannitol, glucitol, citrate, 2 - keto-D-gluconate, or arbutin. With respect to the assimilation of nitrogen compounds, cadaverine (weakly) and D-tryptophan are assimilated, whereas L-lysine, nitrate, nitrite, ethylamine, creatine, creatinine, glucosamine, and imidazole are not. Growth is observed at 30 ° C but not at 35 ° C. Growth in the presence of vitamin-free medium is positive, but growth in the presence of 10 % NaCl plus 5 % glucose, 1 % acetic acid, 0.1 % cycloheximide, and 0.01 % cycloheximide are negative. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are also negative. Additional strains examined. Mexico • Teotihuacan in the State of Mexico (19°41'N, 98°50'W), in the traditional Mexican alcoholic beverage Pulque samples, autumn of 2015, F. L. Hui, NYNU 161114 and NYNU 161129. GenBank accession numbers. Holotype CICC 33274 T (ITS: MF 136070, D 1 / D 2: MF 136062); additional strains NYNU 161114 (ITS: OM 669944, D 1 / D 2: OM 669945) and NYNU 161129 (ITS: OM 669946, D 1 / D 2: OM 669947). Note. Kazachstania paragamospora sp. nov. can be differed from the related three species K. gamospora, K. zonata and K. hellenica by its inability to assimilate trehalose (Table 2). Unlike K. hellenica, the novel species was able to ferment raffinose and grow at 30 ° C but was not able to ferment galactose. Likewise, the novel species did not assimilate maltose and D-galactose. The novel species differed from K. zonata by its inability to assimilate ethylamine, ethanol, and DL-lactate. It differed from K. gamospora in its ability to assimilate L-arabinitol and cadaverine (weakly), and inability to grow in the presence of 10 % NaCl plus 5 % glucose. In all cases, identification by sequencing was the best approach.