DETECTION OF HUMAN ASTROCYTE INFECTION WITH ORTHOHANTAVIRUSES

In this report, a technical protocol for the infection of human astrocytes with the orthohantaviruses Dobrava (DOBV) and Puumala (PUUV) and the detection of infected cells is established. After a 12-day incubation period following infection of the astrocytes, the viruses were quantified in the supernatants. The DOBV and PUUV viruses were stained with monoclonal antibodies and visualized with AEC substrate, with the infected cells stained red.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2024-09-30
DOI
https://doi.org/10.5281/zenodo.13859998
Primary Topic
Viral Infections and Vectors
Type
article
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article

DETECTION OF HUMAN ASTROCYTE INFECTION WITH ORTHOHANTAVIRUSES

Zenodo (CERN European Organization for Nuclear Research)
Viral Infections and Vectors
article

DETECTION OF HUMAN ASTROCYTE INFECTION WITH ORTHOHANTAVIRUSES

article en

Abstract

In this report, a technical protocol for the infection of human astrocytes with the orthohantaviruses Dobrava (DOBV) and Puumala (PUUV) and the detection of infected cells is established. After a 12-day incubation period following infection of the astrocytes, the viruses were quantified in the supernatants. The DOBV and PUUV viruses were stained with monoclonal antibodies and visualized with AEC substrate, with the infected cells stained red.

Zenodo (CERN European Organization for Nuclear Research)
Universidad Luterana Salvadoreña (SV)
Openalex Percentile: Top 53%
Viral Infections and Vectors
DOIOpen Access PDFOpenAlex
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DETECTION OF HUMAN ASTROCYTE INFECTION WITH ORTHOHANTAVIRUSES · Zenodo (CERN European Organization for Nuclear Research) (2024) | TGRS Research Map | TGRS