Buchananella felis sp. nov. isolated from feline pyothorax.

We here describe a novel, Gram-stain positive, catalase positive and oxidase negative bacterium isolated from pyothorax samples of cats. The four described strains 14KM1171, 17KM0085, 22MD1345 and 22MD1512T have a genome size of 2.35-2.40 Mb and a DNA G+C content of 68.3-68.4 mol%. They are genetically most closely related to Buchananella hordeovulneris with a 16S rRNA similarity of 96.9%, digital DNA-DNA hybridization value of 15% and an average nucleotide identity of 73.5%, all clearly below the species threshold. The major cellular fatty acids (C16:0, C15:0 anteiso and C14:0) are the same as in B. hordeovulneris supporting classification in that genus. We therefore propose that our isolates represent a novel species with the name Buchananella felis sp. nov. with type strain 22MD1512T (=CCOS 2118T=DSM 117562T).